Multiplex amplified nominal tandem-repeat analysis (MANTRA), a rapid method for genotyping Mycobacterium tuberculosis by use of multiplex PCR and a microfluidic laboratory chip.

نویسندگان

  • Adam J Merritt
  • Terillee Keehner
  • Lyn C O'Reilly
  • Russell L McInnes
  • Timothy J J Inglis
چکیده

A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 48 10  شماره 

صفحات  -

تاریخ انتشار 2010